Graduated Pipette Tips

Through the introduction of Xiaobian, do a good job of quality control, I believe that your WB data is also appropriate. When your positive control fails to signal, you know that the method may be problematic. For proteins with low expression of the target protein, low-expression internal parameters such as Lamin B1 and HSP60 can be selected protein. However, in the latter, they do not overlap, and it is easy to see how changes in protein concentration affect the band strength of one protein without affecting the band strength of another protein. The four channels are superimposed and the total protein (AzureRed staining) is shown in gray; tubulin-red, \u0026 szlig; -actin-blue and GAPDH-green..

By evaluating the entire protein range, a wider dynamic range is generated. Where the first detection linear range overlaps, the quantification of proteins in this overlapping region will be good. AzureRed is ideal for staining applications, including post-transfer staining to confirm protein transfer from gel to membrane, and quantification of proteins. These stains include gel and membrane stains, and can be imaged in a variety of different ways. When loading, be sure to add an equal amount of protein sample to ensure a fair comparison to minimize loading errors.

Negative controls are also valuable, often not producing target lysates or tissue extracts. Internal control: There are several widely available control antibodies for loading, such as GAPDH, actin or tubulin. By just adding the secondary antibody to incubate the blot, you will be able to clearly see non-specific bands due to the secondary antibodies used in the experiment. In this case, the quantification is inaccurate. Protein samples:

After preparing the Graduated Pipette Tips, use BCA or Bradford and other detection methods to carefully measure the protein concentration. If you are using a recombinant protein, a positive control containing an endogenous form of the target is also a good idea. Performing these checks not only gives you confidence in the progress of your experiment, many journals and magazines now recommend the use of total protein quantification for quality control to demonstrate data repeatability and accuracy. No primary antibody added: This is a simple but often forgotten WB blot control.

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